Electroporation – Sky Blue Microsystems GmbH

Electroporation 

Electroporation is the application of an electric field that causes a brief breakdown of cell membranes to allow molecules to enter the cell. 

A primary biomedical application of electroporation is the reintroduction of engineered T-cells for immunotherapy. 

CAR T-Cells 

A Chimeric Antigen Receptor (CAR) is a receptor designed in the laboratory to bind to certain proteins on cancer cells. When introduced to a T-cell, they bind to the cancer cell such that the T-cell can attack the cancer. Electroporation is an effective means of re-introducing the CAR-T-cell to the body. In sufficient quantities, these CAR-T-cells will find and kill tumour cells. 

Eagle Harbor Technologies: electroporation is a reliable approach that can significantly improve the delivery and duration of interested DNA into the cell.
Electroporation is a reliable approach that can significantly improve the delivery and duration of interested DNA into the cell.

EHT BiPolar microsecond pulse generators (BMP) for electroporation 

Cells respond to electroporation depending on the cell diameter, DNA concentration, temperature, and the electroporation buffer.  

Electroporation is optimized by altering the wave form, the field strength, the pulse length, the gap between the electrodes, and other parameters. 

EHT BiPolar microsecond pulse generators (BMP) offer high voltage, square pulses with fast rise times, and are therefore ideally suited to electroporation. 

Eagle Harbor Technologies: a lively cell is exposed in the external pulse electric field then the cell membrane resealing happens over a range of minutes after the strength of external field dropping down to the threshold voltage.
The principle of electroporation: a lively cell is exposed in the external pulse electric field. 
Eagle Harbor Technologies: the figure shows principle of application of electroporation in the biomedical field: A – General reversible electroporation principle, B – Electrochemotherapy, C – Electrogenetherapy, D – Irreversible electroporation.
Eagle Harbor Technologies: the figure shows principle of application of electroporation in the biomedical field:
A – General reversible electroporation principle,
B – Electrochemotherapy,
C – Electrogenetherapy,
D – Irreversible electroporation.